abn2 [2019-08-13 09:04:08]

endo-1,5-alpha-L-arabinosidase

Locus: BSU_39330

Isoelectric point: 7.37

Molecular weight: 52.38 kDa

Protein length: 469 aa

Gene length: 1410 bp

Function: arabinan degradation

Product: endo-1,5-alpha-L-arabinosidase

Essential: no

Synonyms: yxiA

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of arabinan/ arabinose/ arabitol
• Groups of genes → Secreted proteins

Gene

Coordinates: 4,039,466 4,040,875

The protein

Protein family

• glycosyl hydrolase 43 family (according to UniProt)

Kinetic information

• With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50C. PubMed

Domains

• N-terminal catalytic domain with a characteristic -propeller fold and a C-terminal domain whose function is unknown PubMed

Cofactors

• Ca²⁺ PubMed

Structure

• 2X8F PubMed

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: abn2
  Description: PubMed

  Sigma factors

  • SigH: sigma factor, PubMed, in SigH regulon
  • SigA: sigma factor, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, unknown PubMed, in CcpA regulon

  Regulation

  • expression is stimulated by arabinose and pectin and repressed by glucose PubMed

Biological materials

Mutant

• MGNA-B716 (yxiA::erm), available at the NBRP B. subtilis, Japan
• BKE39330 (abn2::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTAAACAATTCGCCTCT, downstream forward: _UP4_TAAGATGGAGAAGCGCCTTC
• BKK39330 (abn2::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTAAACAATTCGCCTCT, downstream forward: _UP4_TAAGATGGAGAAGCGCCTTC

Labs working on this gene/protein

• [http://www.itqb.unl.pt/research/biology/microbial-genetics [Isabel de Sa-Nogueira]], Lisboa, Portugal [homepage]

References

Loading